Figure 3 | Scientific Reports

Figure 3

From: Atypical chemokine receptor ACKR2-V41A has decreased CCL2 binding, scavenging, and activation, supporting sustained inflammation and increased Alzheimer’s disease risk

Figure 3

CCL2 binds with lower affinity to ACKR2-V41A compared to ACKR2-WT in ligand binding assay. (A) ACKR2-WT and ACKR2-V41A transfected cells were incubated with anti-ACKR2 antibody and a separate tube of ACKR2-WT and ACKR2-V41A transfected cells were incubated with AF647-conjugated CCL2. After normalizing the percentage of CCL2 + cells over ACKR2 + cells, there is a significantly lower binding affinity between ACKR2-V41A than ACKR2-WT for CCL2 (two tailed T test; p-value 0.0071) (n = 5; **p < 0.01). (B) The same experiment was repeated using different concentrations of AF647-conjugated CCL2 ligand (ranging from 50 nM to 1200 nM). ACKR2-WT was saturated when 650 nM of CCL2 ligand was used (n = 3), yielding a 1.5 lower Kd value (67.68 nM) than ACKR2-V41A (103.4 nM).

Back to article page