Figure 7

ACKR2-V41A has lower levels of phosphorylated cofilin after activation with CCL2 ligand. The G protein-dependent response of CHO-k1 cells with atypical chemokine ACKR2-WT and ACKR2-V41A receptors were measured by presence of p-cofilin in either receptor. P-cofilin levels were measured after activation with CCL2 for 30 minutes. Representiative western blots are shown on the left and data analysis of three runs are shown on the right. Data was normalized using cofilin. Cells expressing ACKR2-V41A had significantly lower p-cofilin than cells expressing ACKR2-WT (n = 3, two tail T test, p-value 0.0137).