Figure 3
Mutations in 28 PgABCA2 cDNA sequences from Cry2Ab-resistant strain BX-R and Cry2Ab-resistant F1 progeny from BX-R X Bt4-R2. (a) The predicted PgABCA2 protein includes amino (N) and carboxyl (C) termini (purple) and two transmembrane domains (TMD1 and TMD2). Each TMD contains six transmembrane-spanning regions (TM, orange), three extracellular loops (ECL, green), and two intracellular loops (ICL, blue). The two TMDs are connected by a single intracellular loop (ICL3). ICL3 and the C-terminal domain each contain a nucleotide-binding domain (NBD, yellow). (b) Mutations in PgABCA2 cDNAs from BX-R (three clones from each of three individuals: 1, 2 and 5) and resistant F1 progeny from BX-R X Bt4-R2 (4-5 clones from each of four individuals: A1 and A3 from family A, N1 and N3 from family N, see Fig. 2) relative to the susceptible strain APHIS-S (MG637361). Numbers to the right of the decimal point for each individual indicate the clone sequenced. Exon numbers are shown for APHIS-S. Red triangles above bars indicate premature stop codons, which occur in all sequences except 2.12 from BX-R. Colors within bars show mutations: red for deletions, blue for substitutions, green for insertions and deletions, and orange for the single-bp insertion in exon 2 of N1.8 from the resistant F1 progeny.
