Table 1 Fifteen cDNA mutations in PgABCA2 in three Cry2Ab-resistant larvae from the BX-R strain.

cDNA mutation	Codon^a	Exon^b	Type^c	Effect^d	In gDNA^e	Larva.clone^f
c.1090_1234del	364	6	fs	stop at 373	No	2.42, 2.52, 5.11
c.1609del	537	8	fs	stop at 552	No	5.8
c.1622_1623del	541	9	fs	stop at 517^g	No	2.42, 2.52
c.1786_1843del	596	10	fs	stop at 597	No	1.5
c.1832_1850del	611	10	fs	stop at 615	No	5.9
c.1905_2031del	635	11	fs	stop at 662	No	1.6
c.1963del	655	11	fs	stop at 664^g	No	1.40
c.3097_3100del	1033	18	fs	stop at 1027^g,h	No	1.5, 2.42, 2.52
c.2030 A > T	677	11	ms	E677V	Yes	All but 1.6
c.2753 G > C	918	16	ms	C918S	Yes	All
c.2969 T > C	990	17	ms	L990S	Yes	All
c.2975 C > T	992	17	ms	P992L	Yes	All
c.3163 C > A	1055	18	ms	H1055N	Yes	All
c.3517 G > A	1173	20	ms	V1173I	Yes	All
c.3820 A > G	1274	22	ms	S1274G	Yes	All

^aCodon where the mutation occurs in the full-length PgABCA2 cDNA sequence (MG637361.1).
^bExon where the mutation occurs.
^cfs, frameshift caused by deletion; ms, missense (point mutation, single amino acid substitution caused by single bp change).
^dPosition of premature stop codon (first eight mutations) or amino acid substitution (last seven mutations).
^eIndicates if cDNA mutation also occurs in corresponding gDNA.
^fLarva (1, 2 or 5) and clone where mutation occurred (e.g., 2.42 indicates larva 2, clone 42); three clones were sequenced from each larva (total of nine clones).
^gAffected codons in the mutant and wild-type sequence (from “Codon” column) do not match because additional mutations occur upstream of this mutation.
^hPremature stop codon at 1027 in 1.5; at 997 in 2.42 and 2.52 because of deletion upstream of c.3097_3100del.

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