Table 2 Twenty-two cDNA mutations in PgABCA2 in four Cry2Ab-resistant F1 larvae from BX-R X Bt4-R2.

From: Shared and Independent Genetic Basis of Resistance to Bt Toxin Cry2Ab in Two Strains of Pink Bollworm

Previousa

cDNA mutation

Codonb

Exonc

Typed

Effecte

In gDNAf

Larva.cloneg

Neither

c.432_433insT

144

2

fs

stop at 145

Yes

N1.8

Both

c.1090_1234del

364

6

fs

stop at 373

Noh

A1.3, N1.2, N1.5, N1.8, N1.27

Neither

c.2230_2930del

744

13–16

fs

stop at 751

Noi

A1.9

BX-R

c.3097_3100del

1033

18

fs

stop at 997j

Nok

N1.27

Bt4-R2

c.3313_3589del

1105

19–20

fs

stop at 1112j,l

Noh

A1.3, N3.1, N3.3, N3.6, N3.10

Neither

c.3313_3967delinsm

1106

19–23

fs

stop at 897j

Non

A1.9

Bt4-R2

c.3418_3589del

1140

20

fs

stop at 1147°o

Noh

A1.4, N1.2, N1.5, N1.27

Bt4-R2

c.3556_3588delinsm

1186

20

fs

stop at 1220j,p

Non

A3.1, A3.4, A3.6, A3.7, A3.8, N1.8

Neither

c.3840_3967del

1280

23

fs

stop at 1291

Noh

A1.2, A1.7

Neither

c.1454 C > A

485

8

ns

stop at 485

Yes

N3.9

Neither

c.3972 A > T

1325

24

ns

stop at 1240j

Yes

A1.2, A1.7

Neither

c.3590_3715del

1197

21

InF

loss of 42 aa

Noh

A1.2, A1.7

Neither

c.101 C > T

34

1

ms

T34M

Yes

A1.2, A1.7, A1.9

Neither

c.728 A > T

243

3

ms

D243V

Yes

A1.3

Neither

c.1702C > A

568

9

ms

Q568K

Yes

N3.9

BX-R

c.2030 A > T

677

11

ms

E677V

Yes

A1.2, A1.4, A1.7, A1.9, N3.9

BX-R

c.2753 G > C

918

16

ms

C918S

Yes

A1.2, A1.7, N3.9

BX-R

c.2969 T > C

990

17

ms

L990S

Yes

A1.2, A1.7, N3.9

BX-R

c.2975 C > T

992

17

ms

P992L

Yes

A1.2, A1.7, N3.9

BX-R

c.3163 C > A

1055

18

ms

H1055N

Yes

A1.2, A1.7, N3.9

BX-R

c.3517 G > A

1173

20

ms

V1173I

Yes

A1.2, A1.7, N3.9

BX-R

c.3820 A > G

1274

22

ms

S1274G

Yes

A1.2, A1.7, N3.9

  1. acDNA mutation found previously in BX-R (Table 1), Bt4-R2 (Mathew et al. 2018), both, or neither.
  2. bCodon where the mutation occurs in the full-length PgABCA2 cDNA sequence (MG637361.1).
  3. cExon where the mutation occurs.
  4. dfs, frameshift caused by deletion; ns, nonsense mutation (single base-pair substitution that introduces premature stop codon); InF, in-frame deletion; ms, missense (point mutation, single amino acid substitution caused by single bp change).
  5. ePosition of premature stop codon (first 11 mutations), amino acids lost (c.3590_3715del), or amino acid substitution (last 10 mutations).
  6. fIndicates if cDNA mutation also occurs in corresponding gDNA.
  7. gLarva (A1, A3, N1, or N3) and clone where mutation occurred (e.g., N1.8 indicates larva 1 from family N, clone 8); 4–5 clones were sequenced from each larva (total of 19 clones).
  8. hMis-spliced, deletion caused by exon skipping.
  9. iMis-spliced, deletion due to alternative 5′ and 3′ splice sites.
  10. jAffected codons in the mutant and wild-type sequence (from “Codon” column) do not match because additional mutations occur upstream of this mutation.
  11. kMis-spliced, deletion due to alternative 3′ splice site.
  12. lPremature stop codon at 1063 in A1.3 because of deletion upstream of c.3313_3589del.
  13. mSequence corresponding to insertion not shown due to large number of nucleotide bases.
  14. nMis-spliced, indel caused by intron retention.
  15. oPremature stop codon at 1097 in N1.27 and at 1098 in N1.2 and N1.5 because of deletions upstream of c.3418_3589del.
  16. pPremature stop codon at 1172 in N1.8 because of deletion upstream of c.3556_3588delins.
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