Figure 3 | Scientific Reports

Figure 3

From: Single-cell analysis of childhood leukemia reveals a link between developmental states and ribosomal protein expression as a source of intra-individual heterogeneity

Figure 3

Intra-individual transcriptional heterogeneity reveals deregulated genes and pathways within cALL samples. (A) UMAP representation of cALL cells in G1 phase not clustering with healthy cell clusters (n = 16,731). (B) Mean Adjusted Rand Index (ARI) of clustering solutions over a range of resolutions (highest mean ARI at 1.3 resolution). (C) Clusters of cells identified in cALL samples using the highest mean ARI resolution. (D) Proportion of cells belonging to each intra-individual cluster after removing clusters having less than 10% of cells (n = 16,162). (E) Differentially expressed genes between two the clusters of cells within the HHD.1 sample (log fold-change > 0.75 = green, >1 = orange). (F) Heatmap and unsupervised clustering of enriched GO biological pathways obtained using the top 100 most significant differentially expressed genes of cALL samples.

Back to article page