Table 3 Nucleotide sequence of the primer sets used for MBP fusion expression.
From: Development of novel detection system for sweet potato leaf curl virus using recombinant scFv
Primer name | Sequences (5′-3′) |
|---|---|
SPLCV_F7_attB1 | GGG GAC AAG TTT GTA CAA AAA AGC AGG CTT CCT GGT GCA GTC TGG GGG AGG |
SPLCV_F7_attB2 | GGG GAC CAC TTT GTA CAA GAA AGC TGG GTC CTA GGT GAC CTT GGT CCC TCC GC |
SPLCV_G7_attB1 | GGG GAC AAG TTT GTA CAA AAA AGC AGG CTT CGT GCA GTC TGG GGC TGA GGT |
SPLCV_G7_attB2 | GGG GAC CAC TTT GTA CAA GAA AGC TGG GTC CTA GGC TCC AGT CTG CTG ATG GA |
