Figure 1
UGCG overexpression enhances glycolysis and cellular respiration. (A) Substrate oxidation was measured by U-14C-glucose tracing experiments. The evolved 14CO2 was determined. Data are presented as mean of n = 3 ± standard error of the mean (SEM). (B) Representative graph of the oxygen consumption rate (OCR) determined by Seahorse XFe analyzer. (C) ATP production (basal respiration − respiration after oligomycin injection), maximal respiration rate (respiration after N5,N6-bis(2-fluorophenyl)-[1,2,5]oxadiazolo[3,4-b]pyrazine-5,6-diamine (BAM15) injection − respiration after antimycin a/rotenone injection), reserve capacity (respiration after BAM15 injection − basal respiration). Data are presented as mean of n = 3 ± SEM. (D) Anaerobic glycolysis was determined by using 3-3H-D-glucose and measuring the released labelled H2O. Data are presented as mean of n = 3 ± SEM. (E) Representative graph of the extracellular acidification rate (ECAR) quantified by Seahorse XFe analyzer. (F) Glycolytic capacity (deduced from ECAR after oligomycin treatment − basal ECAR). Data are presented as mean of n = 3 ± SEM. (G) Energy map of MCF-7/UGCG OE and control cells. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001.
