Figure 3
Schematic figures of the PCR products from chromosome Z and two polymorphic clones from chromosome W, WL and WS clones. The intronic regions origin at GT and end at AG as in GT-AG rule of introns. The estimated length of introns in CHD-Z, CHD-WL and CHD-WS, amplified with the primers made in this study, CHD1-F1/R1, were 312, 203 and 171 bp, respectively. Polymorphic region of the intron of GHD1 genes on chromosome Z and W of Japanese murrelet. The region containing a polymorphic intron divided into five areas, A to E. Comparison between CHD-WL and CHD-WS indicated that the DNA sequences of this region were coincided perfectly with two exception with the copy number of a-32 repeat and one nucleotide change in region A. On the other hand, comparison between CHD-Z and CHD-W, the region C exites in only CHD-Z. The regions of A and E have more than 70% homology, however, B involved repeat region and D region in a-32-bp-repeat have lower homology than regions of A and E.
