Figure 3

Bbs2^−/− retinas have an increased number of ectopically localized ribbon synapses. P21 retinas were probed with an anti-RIBEYE antibody to identify ribbon synapses along with biotinylated-PNA to visualize cone outer segments and pedicles (a,b). Ectopically localized ribbon synapses within the outer nuclear layer are observed in Bbs2^−/− mice (d), but not in control littermates (c). The number of ectopically localized synapses is significantly elevated in Bbs2^−/− mice (e).