Figure 4
Sirolimus treatment reduced glomerular damage and cell proliferation at day 4 in experimental FSGS. (A) Sirolimus treated mice showed significant less affected glomeruli compared to phosal treated mice. At least 55 glomeruli per mouse were scored. Sirolimus treated mice n = 11, phosal treated mice n = 10. (B) Proliferation is expressed as ki-67 positive cells per glomerulus. Ki-67 positive cells were counted in at least 55 glomeruli per mouse. Cell proliferation in the tuft and in the Bowman’s space was significantly reduced at day 4. Sirolimus treated mice n = 12, phosal treated mice n = 10. (C, E) Representative images of glomeruli stained with PAS of phosal and sirolimus treated mice. Arrows indicate vacuolization in the glomerulus. (D, F) Representative images of glomeruli stained with ki-67 (brown colour) and PAS without hematoxylin stain. Arrows indicate cell proliferation in the Bowman’s space, arrowheads indicate cell proliferation in the glomerular tuft. Mean with SEM is shown. *P ≤ 0.05, ***P ≤ 0.001.
