Figure 2

Knockout mutants of MpPRR, MpRVE and MpTOC1 affects each other’s expression. qRT-PCRs measuring expression of M. polymorpha clock genes during two consecutive days of constant light in wild type (Tak1), Mpprr^ko, Mprve^ko and Mptoc1^ko. Plants were entrained in ND and sampling was conducted every six hours starting 24 hours after switch to LL. Graphs show the average expression of MpPRR (a), MpTOC1 (b) and MpRVE (c) in three replicates of wild type or mutant line for each time point. Green and orange lines combined with filled symbols indicate significantly overall higher or lower expression as compared to wild type (bold line), respectively. Small symbols indicate the value of each replicate.