Figure 3

MpTAA expression levels as well as levels of free IAA display circadian oscillations that are disrupted in knockout mutants of Marchantia polymorpha clock genes. (a,b) qRT-PCRs measuring expression of M. polymorpha auxin biosynthesis genes during two consecutive days of constant light in wild type (Tak1), Mpprr^ko, Mprve^ko and Mptoc1^ko. (a) MpTAA expression. (b) MpYUC2 expression. (c) IAA measurements of whole wild type (Upp14) and Mplux^ge-9 gemmalings in LL over 32 hours. (d) MpPRR expression in the Mplux^ge-9 and Mplux^ge-19 lines compared to wild type (Upp1 and Upp5). For qRT-PCR experiments plants were entrained in ND and sampling was conducted every four (d) or six (a,b) hours starting 12 (d) or 24 (a,b) hours after switch to LL. qRT-PCR graphs show the average expression of three replicates of wild type or mutant line (a,b), or four replicates (two for each of the genotypes) (d), for each time point. Green and red lines combined with filled symbols indicate significantly overall higher or lower expression as compared to wild type (bold line), respectively. Small symbols indicate the value of each replicate.