Figure 4
Manipulation of auxin levels, distribution and response affects nyctinastic movements. (a) Gemmalings growing on different concentrations of IAA. (b) Gemmalings growing on different concentrations of BUM. (c) Plot of growth angle change over time for gemmalings growing on different concentrations of IAA. (d) Plot of growth angle over time for gemmalings growing on different concentrations of BUM. Angle was measured as indicated in (a). Plants (a–d) were entrained in ND for three to five days, then transferred to IAA (a,c) or BUM (b,d) for one and three days in ND, respectively, before transfer to LL. (e,f) Average amplitude of nyctinastic movement for various genotypes (e) and for wild type (Upp5) growing on L-Kyn (f). WT (wild type) M (male) and F (female) (e) are of the Australian accession39. Experiments (e,f) were performed using younger gemmalings in (f), which is why the controls are not identical. Graphs (e,f) shows means ± SE of three replicates. **P < 0.01, ***P < 0.001 for two-tailed t-test against control (wild type or mock).
