Figure 2

Electrophysiological activity of rat native synaptic receptors is preserved by low temperature. (a) Ion current responses of oocytes microtransplanted with rat synaptic receptors measured by Two-Electrodes Voltage Clamp. (b–c) Maximum current of synaptic receptors normalized to the control (PMI = 0). The currents generated by GABA and glutamate receptors, across PMIs time points, are well preserved at 2 °C but not at 21 °C showing significant differences compared to the control (time 0) (N = 7–11 oocytes per point, for a detailed description of the number of oocytes in each point, please see supplementary Table 2). Black and green lines represents linear regressions and one-phase decay fits, respectively. (d) The E/I ratio (kainate/GABA current) was calculated only in oocytes that have a clear signal for both GABA and kainate currents. Data shown is the mean  ± SEM of 7–10 oocytes per point; please see supplementary Table 1). No differences were observed across groups. Statistical analysis was done using one-way ANOVA test, followed by Dunnett’s multiple comparison test where appropriate. Statistical differences where *p < 0.05, **p < 0.01; ***p < 0.001; **** p < 0.0001). The mean values of the E/I ratio were fit with a linear regression (black) and a third order polynomial equation (green).