Figure 4
From: G-CSF Inhibits Pulmonary Fibrosis by Promoting BMSC Homing to the Lungs via SDF-1/CXCR4 Chemotaxis
G-CSF preconditioning promoted BMSC migration via the SDF-1/CXCR4 axis in vitro. (A) The expression levels of VLA-4, ICAM-1, VCAM-1 and CXCR7 were quantified by real-time PCR. (B) The expression of CXCR4 was quantified by real-time PCR and FCM at 24 hours after G-CSF treatment. (C) The proliferation of BMSCs with or without G-CSF (30 ng/mL) was tested by CCK-8 assay. (D) Experimental design of the transwell assay: lungs collected from control and BLM mice were cut into fragments and placed in the lower transwell chamber. In the upper compartment of the transwell chamber, GFP-labelled BMSCs with or without G-CSF (30 ng/mL) pretreatment were seeded to test chemoattraction. (E) Representative image of migrated GFP-labelled BMSCs (green). (F) Analysis of GFP-labelled BMSC migration. (G) The mRNA and protein expression of SDF-1 in control and BLM-treated lungs as quantified by real-time PCR and Western blotting. n = 3. *P < 0.05, **P < 0.01 between the indicated groups. Bars: mean ± SD.
