Figure 5
Effect of HC-030031 (HC) or its vehicle (Veh) on expression of TRPA1, TRPV1 and TRPV4 mRNA in lumbosacral DRGs (a–c) and TGs (d) isolated from control (Con) mice and mice treated with DSS for 7 days. HC (100 mg/kg, i.p.) or its vehicle was injected one hour prior to sacrifice of mice. Values are expressed as means + SEM (n = 4–5). Two-way ANOVA did not show any DSS × HC interaction and any HC effect but revealed a DSS effect on the expression of TRPA1 mRNA (aP < 0.05). (e) typical traces and pooled data of the inward currents evoked by AITC and capsaicin (CPS) in thoracosacral DRG neurons isolated from control (Con) mice and mice treated with DSS for 7 days. ΔI refers to the difference between current amplitude measured at -60 mV before and after application of AITC/CPS. The inward currents are shown as current density pA/pF. Values are expressed as means - SEM (n = 16 neurons from 3 mice/group). Mann-Whitney U test demonstrated a significant difference between the AITC responses of the Con and DSS groups (*P < 0.05).
