Figure 2 | Scientific Reports

Figure 2

From: Signal profiling of the β1AR reveals coupling to novel signalling pathways and distinct phenotypic responses mediated by β1AR and β2AR

Figure 2

Gαs activation and cAMP production induced by the β1AR. (a) Schematic representation of the Gαs-Rluc/Gγ1-GFP biosensor used to study the Gαs induced β1AR signalling. (b) Schematic representation of the cAMP biosensor used to study the cAMP increase induced by the β1AR Gαs activation. HEK 293 cells were transfected with (c,e) Gαs-Rluc, Gγ1-GFP and untagged Gβ1 or with the (d,f) EPAC biosensor, along with the β1AR. Kinetic curves represent time course of (c) Gαs activation (vehicle and isoproterenol, n=3) or (d) cAMP accumulation (vehicle and isoproterenol, n=3) expressed as absolute BRET ratio. Concentration-responses curves were generated for (e) Gαs activation and (f) cAMP accumulation following β1AR activation by the indicated ligands. Data were normalized to maximal isoproterenol response, which was taken as 100%, and are expressed as mean ± SEM values. Detail of the number of experiments, maximal responses, pEC50 values and statistical comparisons of curve parameters for different ligands are provided in Supplementary Tables S1 and S2.

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