Figure 8 | Scientific Reports

Figure 8

From: Signal profiling of the β1AR reveals coupling to novel signalling pathways and distinct phenotypic responses mediated by β1AR and β2AR

Figure 8

β-arrestin2 recruitment by β1AR. (a) Schematic representation of the βarr2 biosensor. HEK 293 cells were transfected with β1AR-GFP along with β-arrestin2-Rluc. (b) Kinetic curves represent time course of β-arrestin2 recruitment (vehicle and isoproterenol n = 3) expressed as absolute BRET ratio. (c) Concentration-responses curves for β-arrestin2 recruitment following β1AR activation by indicated ligands. Data were normalized to maximal isoproterenol response, which was taken as 100%, and are expressed as mean ± SEM values. Detail of the number of experiments, maximal responses, pEC50 values and statistical comparisons of curve parameters for different ligands are provided in Supplementary Tables S1 and S2.

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