Figure 12 | Scientific Reports

Figure 12

From: Ultra-rapid glutathionylation of chymotrypsinogen in its molten globule-like conformation: A comparison to archaeal proteins

Figure 12

Average pKa determination of SsPDO. rSsPDO (1 µM) was reacted with CDNB (1 mM) at variable pH values (red line). Average pKa = 8.6 ± 0.1 of the three reactive cysteines in rSsPDO. As a control experiment, GSH (100 µM) was reacted with CDNB (1 mM) at variable pH values (gray line). The error bars represent the S.D. from three independent experiments. The theoretical curve (black line) of an unperturbed protein cysteine (pKa = 9.1) is also reported.

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