Figure 4

Pancreatic islet assessment. (A) Representative immunofluorescent microphotographs of IR^+/+ (Wildtype) and IR^f/f (postnatal-OIRKO) male islets stained for Insulin (Ins, green), Glucagon (GCG, red) and Dapi (blue). Scale bar = 100 μm. (B) Morphometric analysis of pancreatic islets: mean islet area (left panel) and islet circularity (right panel) assessed with Zen2.3 software, in n = 60–85 islets, in n = 3 mice/group (M, male; F, female). A circularity value of 1.0 indicates a perfect circle. As the value approaches 0.0, it indicates an increasingly elongated polygon. Data are expressed as mean ± SEM.