Figure 6
The effect of DNA-LL37 complexes stimulation on the antigen presentation capacity of pDCs of T1D and HC subjects. Following stimulation with DNA-LL37 complex (3 µg complex per 100 µL of media), pDCs were assessed for the expression of markers of antigen presentation (HLA-DR) and costimulation (CD80, CD86) and compared to unstimulated pDCs (MC) within the groups and between the T1D (n = 24) and HC (n = 15) groups. (a) Frequency of CD80 + pDCs, (b) MFI of CD80 on pDCs, (c) Frequency of CD86 + pDCs, (d) MFI of CD86 on pDCs, (e) Frequency of pDCs co-expressing CD80 and CD86, (f) Frequency of ILT7 expressing pDCs, (g) Frequency of HLA-DR expressing pDCs and (h) MFI of HLA-DR on pDCs. The number of subjects analysed for HLA-DR expression were; T1D (n = 15) and HC (n = 12). Data is presented as mean (±SEM) frequency or MFI, and one-way ANOVA followed by Tukey’s multiple comparison test was used to compare the means. P < 0.05 was considered statistically significant. Relevant FMO tubes were used to set gates.
