Figure 9
The activation of CD4 + T cells co-cultured with autologous pDCs stimulated with DNA-LL37 complexes. (a) Following the co-culture of CD4 + T cells with DNA-LL37 complex stimulated (Stim) and unstimulated (MC) autologous pDCs (3 µg complex per 100 µL of media), we assessed the expression of, (a) CD69 and b) CD71 on CD4 + T cells of all subjects. (c, d) Activation status of CD4 + T cells in the T1D (n = 16) and HC (n = 12) subject groups was assessed by determining the frequency of CD4 + T cells expressing CD69 and CD71. Data is presented as mean ± SEM and Student’s t test was used to compare the means in figures a and b whereas, one-way ANOVA followed by Tukey’s multiple comparison test was used to compare the means in figures c and d. P < 0.05 was considered statistically significant. Relevant FMO tubes were used to set gates.
