Figure 2
Bacteriophage K1F infection of E. coli EV36. (A,B) Growth of E. coli EV36 (A) and E. coli MG1655 (B) cultures infected by phages K1F, K1F-GFP or T7 at a MOI of 0.001 in comparison with uninfected control cultures, NTC (+/− SD, n > 3). (C–E) Analysis of bacterial cell death in phage-infected cultures by flow cytometry; E. coli EV36 cultures were infected with phage at a MOI of 0.001; NTC = uninfected control (C) Sytox Green Dead Cell Stain MFI (+/− SD, n = 3) of phage K1F infected E. coli EV36. (D) Propidium iodide (PI) MFI (+/− SD, n = 3) of phage K1F infected E. coli EV36. (E) Flow cytometry histogram of phage K1F-GFP infected E. coli EV36 at 0 min following infection (grey) or 120 min (green). (F) GFP MFI (+/− SD, n = 3) of phage K1F-GFP infected E. coli EV36; NTC = uninfected Control. (G) Negative staining EM images of sample (Gold-conjugated GFP binding probe with phage K1F-GFP) and controls. Wild-type control is represented by phage K1F incubated with the probe; Gold control was performed using only the 5 nm gold incubated with phage K1F-GFP.
