Figure 8
Temporal impedance profiling as measured using the xCELLigence system (A–F). Graphs displaying the barrier resistance over time of hCMEC cultures treated with phage K1F at a concentration range of 105 to 109 PFU/ml (A,B), E. coli EV36 at a concentration range of 103 to 107 CFU/ml (C,D), or combination treatment of 107 CFU/ml E. coli and 104 PFU/ml phage K1F added 1 h before-, simultaneously-, or 1 h after bacterial addition (E,F). NTC = Untreated hCMEC cultures. X-axis show time from cell seeding. Vertical lines denote the addition of treatment at 0 h, and 24- and 72 h post treatment. The data is presented as the average normalised cell index across the acute (24 hours) and long-term (72 hours) incubate period (+/− SD, n > 3).
