Table 2 Overview of statistical models with sample sizes.

From: Good performance of turquoise killifish (Nothobranchius furzeri) on pelleted diet as a step towards husbandry standardization

Compared parameter (statistical method)

Model

Sample size per dietary group or combination

Sex-specific sample size per group

Sample size per tank

Total sample size

SL 15 days (LME)

SL15 ~ diet + (1|tank)

90

90 J

30 J

180

SL 30 days (LME)

SL30 ~ diet + (1|tank)

32

16 M:16 F

4 M:4 F

64

SL 56 days (LME)

SL56 ~ diet + (1|tank)

32

16 M:16 F

4 M:4 F

64

BM 15 days (LME)

BM15 ~ diet + (1|tank)

90

90 J

30 J

180

BM 30 days (LME)

BM30 ~ diet + (1|tank)

32

16 M:16 F

4 M:4 F

64

BM 56 days (LME)

BM56 ~ diet + (1|tank)

32

16 M:16 F

4 M:4 F

64

SGR (Gaussian LM)

SGR ~ diet + sampling point

7

14

Fulton’s condition (LME)

K ~ diet + sex + (1|tank)

32

16 M:16 F

4 M:4 F

64

VFS (MPA)

VFS ~ diet + error (tank)

32

16 M:16 F

4 M:4 F

64

LM (LME)

LM ~ diet + BMdis + (1|tank)

32

16 M:16 F

4 M:4 F

64

Additional LM (LME)

LM ~ diet + HVE + BMdis + (1|tank)

19-21

10 M:10 F

2-3 M:2-3 F

40

HVE (LME)

HVE ~ diet + sex (1 | tank)

20-21

10 M:10-11 F

2-3 M:2-3 F

41

THCV

Pearson’s χ2 test

19-21

10 M:10 F

2-3 M:2-3 F

40

N of eggs (Neg.Bin. GLMM)

N eggs ~ pair combination + BM + (1|tank)

16

16 M:16 F

4 M:8 F

32/2358

FR (Bin. GLMM)

FR ~ pair combination + (1| TM.TF)

16

16 M:16 F

4 M:8 F

32/2358

Ovary mass (LME)

OM ~ diet + BMdis + (1|tank)

16

16 F

4 F

32

Egg diameter (LME)

ED ~ diet + (1|tank/ID.female)

16

16 F

4 F

32/837

30dpf egg survival (Bin. GLMM)

ES ~ diet + (1|dish)

100

400

30 dpf egg diapause stage

Pearson’s χ2 test

25-31

114

  1. TM.TF is combined factor made by joining tank identity of male and tank identity of female.
  2. The number behind the slash symbol is total number of eggs analysed. Models with missing sex factor were performed separately for each sex due to significant sex-specific interaction (or only completed for females in the case of reproductive parameters). LME – Linear mixed effect model56; GLMM – Generalized linear mixed effect model56. In all binomial models raw data were used instead of percentages to account for sample size. MPA – Multistratum permutation analysis with specified 10000 iterations57. χ2 analyses were completed by Pearson’s chi-squared test with simulated p-value (based on 10000 replicates). J: juvenile; M: male; F: female. For parameter coding refer to “Experimental procedures”.