Figure 4
From: Analysis of cell-associated DENV RNA by oligo(dT) primed 5’ capture scRNAseq
Characterization of negative-sense DENV RNA by 5′ capture scRNAseq (A) Fraction of indicated cell populations containing either only positive-sense DENV RNA (black), both positive- and negative-sense DENV RNA (gray), or only negative-sense DENV RNA (white) as quantified by 5′ capture scRNAseq. (B) Percentage of all DENV-mapped RNA with a negative-sense orientation in DENV-1 infected (blue) or sorted NS1pos DENV-1 infected cells as quantified by 5′ capture scRNAseq. (C) Relationship between positive-sense and negative-sense DENV RNA abundance within DENV-1 infected cells. (D) Aggregated negative-sense DENV-1 genomic coverage and sequence depth of in vitro infected samples as assessed by 5′ capture scRNAseq.
