Figure 1
From: Identification of two aptamers binding to Legionella pneumophila with high affinity and specificity
Schematic illustration of bacterial cell-SELEX procedure used in this study. A random library of oligonucleotides is incubated with Lp lp120292 at room temperature for 1 h. Sequences that do not bind are washed off and the cell-bound sequences are then released and amplified via PCR. The resulting sequences are then submitted to another round of positive selection. P. fluorescens and P. putida were used to perform counter-selection rounds to eliminate non Lp-specific sequences.
