Figure 2
From: Identification of two aptamers binding to Legionella pneumophila with high affinity and specificity
Fluorescent labeling of Lp with FITC-labeled aptamers pools obtained after selected round of SELEX. Lp strain lp120292 was incubated without aptamers (Lp, a), with 35 nM of the aptamer library (Lp-lib, d) and with the aptamer pools obtained after round 1 (Lp-R1, e), 6 (Lp-R6, f), 7 (Lp-R7, g), 8 (Lp-R8, h) and round 10 (Lp-R10, i). The fluorescence obtained with aptamer from round 10 alone (R10, b), without cells, was also evaluated. Lp*GFP is a GFP producing version of lp120292 and is used as a positive control (c). The percentages refer to the proportion of cells with fluorescence above the autofluorescence, falling in the Green_Lp region.
