Figure 3 | Scientific Reports

Figure 3

From: A unique hybrid characteristic having both pro- and anti-inflammatory phenotype transformed by repetitive low-dose lipopolysaccharide in C8-B4 microglia

Figure 3

Neuroprotective molecules such as Ntf5, Gipr, and Ccl7 highly expressed in REPELL-microglia. C8-B4 microglia were treated with low-dose LPS (1 ng/mL) one or three times (n = 3, in triplicate), and relative mRNA expression of neuroprotective genes was measured by real-time RT-PCR using the 2−∆∆Ct method 4 h after the last LPS treatment. Data were normalized to GAPDH and expressed as the relative fold change over untreated cells. (a) Neurotrophic molecules, (b) incretin receptors, and (c) cell surface receptors associated with neuroprotection. Mean ± SE of each group are shown. Data are representative of three independent experiments. LPS x0, no treatment; LPS x1, single treatment with low-dose LPS; LPS x3, treatment with low-dose LPS three times every 24 h. *p < 0.05 for one-way ANOVA with Tukey’s post-hoc correction for multiple comparisons. n.s., not significant.

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