Figure 4 | Scientific Reports

Figure 4

From: A unique hybrid characteristic having both pro- and anti-inflammatory phenotype transformed by repetitive low-dose lipopolysaccharide in C8-B4 microglia

Figure 4

Maintaining promotion of phagocytic activity in REPELL-microglia. C8-B4 microglia were treated with low-dose LPS (1 ng/mL) one or three times (n = 3, in triplicate). At 24 h after the last LPS treatment, cells were incubated with fluorescent latex beads for 3 h at a cell:bead ratio of 1:5. The phagocytosis rate (left panel) and mean fluorescence intensity (MFI) (right panel) were assessed using a Beckman Coulter Gallios flow cytometer with Kaluza software. The MFI of cells phagocytizing beads was expressed as the relative fold change over untreated cells. Mean ± SE of each group are shown. Data are representative of three independent experiments. LPS x0, no treatment; LPS x1, single treatment with low-dose LPS; LPS x3, treatment with low-dose LPS three times every 24 h. *p < 0.05 for one-way ANOVA with Tukey’s post-hoc correction for multiple comparisons.

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