Figure 3

Influence of capsaicinoids on β-secretase. (a) Protein levels of BACE1 after incubation of SH-SY5Y APP⁶⁹⁵ cells with capsaicin, natural compound and DHC examined by WB (n ≥ 3). Representative signals are illustrated on the right. (b) BACE1 gene expression analysis by RT-PCR after capsaicinoid treatment in SH-SY5Y cells (n ≥ 4). (c) β-secretase activity in living cells (n = 10) and in isolated membranes (n = 3) of capsaicin and natural compound incubated SH-SY5Y wt cells. Error bars represent the standard error of the mean. Asterisks show the statistical significance calculated by unpaired Student’s t test or ANOVA (*p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001).