Figure 3
Modulation of cardiomyogenic potential of HH24 embryonic epicardium and HH17 PE/ST in hanging drops by microRNA mimics administration. Modulation of microRNAs by Bmp and Fgf signaling. Panel A represents qPCR analyses of HH24 embryonic epicardium treated with microRNA mimics. Observe that miR-195 and miR-223 selectively enhance expression of terminally differentiation markers such as Tnnt2 and Mhy15. Panel B represents qPCR analyses of HH17 PE/ST explants cultured in handing drops and treated with microRNA mimics. Observe that miR-195 and miR-223 selectively enhance expression of terminally differentiation marker Tnnt2, while administration of miR-100 does not changes it expression. Panel C qPCR analyses of HH17 PE/ST explants cultured in handing drops and treated with Bmp2 and Fgf2, respectively. Observe that Bmp2 selectively enhances expression of miR-195 while no significant differences are observed after Fgf2 administration. HH17 PE were dissected from >30 embryos, treated with the corresponding microRNA mimics and/or Bmp/Fgf treatment, respectively, and subsequently pooled to perform RNA isolation. On each case, three-to-five distinct biological replicates were subsequently tested by qPCR.
