Figure 4

Potential targets driving PE/ST explants cardiomyogenesis. Panel A. Venny diagram of shared potential targets of miR-146, miR126, miR-195 and miR-223. Observe that only 58 potential target (representing 1%) were shared between these four microRNAs. Panels B-H. qPCR analyses of the expression of Wnt5a (panel B), Smurf1 (panel C), Sema5a (panel D), Smad3 (panel E), Foxp1 (panel F), Fols2 (panel G) and RhoV (panel H) in HH17 hearts, HH17 control PE/ST explants and PE/ST explants treated with miR-100, miR-125, miR-146, miR-195 and miR-223. Observe that over-expression of miR-195 increases expression of Wnt5a, Smurf1, Smad3, Foxp1, Fols2 and RhoV, while no significant differences were observed for Sema5a. On the contrary, miR-100 over-expression lead to significant downregulation of Wnt5a, Smurf1, up-regulation of Smad3 and no significant differences of Sema5a, Foxp1, Fosl2 and RhoV. HH17 PE were dissected from >30 embryos, treated with the correspoding microRNA mimics and subsequently pooled to performed RNA isolation. On each case, three distinct biological replicates were subsequently tested by qPCR.