Figure 4
From: Rational Design of a Protein Kinase A Nuclear-cytosol Translocation Reporter
Specificity of PKA kinase reporter to PKA activation by pharmacological and genetic inhibition. (A) Nuclear-cytosol translocation of PKA kinase reporter by FSK/IBMX treatment and reversal with PKA inhibitor Rp-cAMPS (500 μM, Santa-Cruz, Sc-24010). (N = 3 experiments, 45 cells). (B) Nuclear-cytosol translocation of PKA kinase reporter in cells co-expressing mCherry-PKI. (N = 3 experiments, 54 cells). (C) Nuclear-cytosol translocation of PKA kinase reporter in cells co-expressing mCherry. (N = 3 experiments, 51 cells).
