Figure 6
From: Rational Design of a Protein Kinase A Nuclear-cytosol Translocation Reporter
Duplex monitoring of Ca2+ and PKA in cells followed by isoprenaline and FSK/IBMX treatment. (A) Ca2+ response as reported by R-GECO to isoprenaline (100 μM, Sigma-Aldrich, I5627-5G) and FSK/IBMX treatment in cells also expressing PKA kinase reporter and nucleus marker. (N = 3 experiments, 48 cells) (B) Nuclear-cytosol translocation response of PKA kinase reporter in same cells in (A). (C) Duplex monitoring of Ca2+ and PKA activity from (A) and (B).
