Table 2 Parameters derived from the RT-qPCR standard curve method for the primer pairs used in this study.
Gene Symbol | Intercept | Slope | PCR Amplification Efficiency | Regression Coefficient (R-squared) |
|---|---|---|---|---|
ACTB | 15.44 | 3.491 | 93.4 | 0.9996 |
B2M | 14.09 | 3.482 | 93.7 | 0.9995 |
CASC3 | 21.88 | 3.519 | 92.4 | 0.9994 |
GAPDH | 14.72 | 3.574 | 90.4 | 0.9993 |
HMBS | 22.85 | 3.459 | 94.6 | 0.9990 |
HPRT1 | 23.18 | 3.535 | 91.8 | 0.9987 |
IPO8 | 22.38 | 3.579 | 90.3 | 1.0000 |
PPIB | 19.16 | 3.510 | 92.7 | 0.9977 |
RPLP0 | 16.72 | 3.435 | 95.5 | 0.9983 |
SDHA | 21.74 | 3.566 | 90.7 | 0.9996 |
UBE2D2 | 20.12 | 3.378 | 97.7 | 0.9996 |
18S rRNA | 4.95 | 3.583 | 90.1 | 0.9957 |
IFNG | 19.36 | 3.520 | 92.3 | 0.9992 |
