Figure 5
Autophagy activity is increased following starvation for 24 hours. (A) Representative electron micrographs of Purkinje cells from PK-LC3 mice. Left image shows a part of the perikaryal region of Purkinje cells of the mice before starvation, while right image exhibits that after starvation. White arrows show lysosomes or autolysosomes, while black arrows display autophagosomes with double membranes. Scale bar corresponds to 500 nm. (B) Graphs of the volume densities for autophagy-related structures including autophagosomes and autolysosomes/lysosomes (right panel), autophagosomes (central panel), and autolysosomes/lysosomes (left panel). Left side graph corresponds to all autophagy-related structures. Center graph corresponds to autolysosomes only. Right side graph corresponds to lysosomes and autolysosome structures. The results are expressed as the mean ± SEM. *P < 0.05 (n = 3).
