Figure 4

The effect of IS on regional 5HT (a), 5HIAA levels (b), and 5HT turnover (c); n = 45. (a) Data are presented as the mean ± SD due to passed normality test (alpha=0.05). Ordinary ANOVA test has been used to compare the data. B2 – Anova – 0.0005 / F (DFn, DFd) = F (2, 42) = 9.305; Student t-test: **CON vs 100 IS P = 0.0057; **CON vs 200 IS P = 0.0035. All Controls Anova – <0.0001 / F (DFn, DFd) = F (4, 70) = 20.01. All 100 IS Anova – <0.0001 / F (DFn, DFd) = F (4, 70) = 31.13. All 200 IS Anova – <0.0001 / F (DFn, DFd) = F (4, 70) = 24.43. (b) Data are presented as the mean ± SD due to passed normality test (alpha=0.05). Ordinary ANOVA test has been used to compare the data. B2 – Anova – 0.0012 / F (DFn, DFd) = F (2, 42) = 7.965; Student t-test: **CON vs 100 IS P = 0.0063; **CON vs 200 IS P = 0.0027. B3 - Anova – 0.0235 / F (DFn, DFd) = F (2, 42) = 4.105; Student t-test: *100 IS vs 200 IS P = 0.0148. All Controls Anova – <0.0001 / F (DFn, DFd) = F (4, 70) = 27.59. All 100 IS Anova – <0.0001 / F (DFn, DFd) = F (4, 70) = 40.52. All 200 IS Anova – <0.0001 / F (DFn, DFd) = F (4, 70) = 52.14. (c) Data are presented as a median (full range) due to unmeet normal distribution condition (alpha=0.05). Data were analyzed using Kruskal-Wallis test. All 100 IS Kruskal-Wallis test– 0.0229 / Kruskal-Wallis statistic 11.35. All 200 IS Kruskal-Wallis test – 0.0052 / Kruskal-Wallis statistic 14.78. B1 – cerebellum; B2 – brainstem; B3 – cerebral cortex; B4 – hypothalamus; B5 – striatum with hippocampus; 5HT – serotonin; 5HIAA – 5-hydroxyindolacetic acid; 5HIAA/5HT ratio – 5HT turnover; CON – control group; 100 IS – group receiving IS in the dose of 100 mg/kg b.w./day; 200 IS – group receiving IS in the dose of 200 mg/kg b.w./day; IS – indoxyl sulfate.