Figure 5

Primary culture cells possess self-renewal capacity. (a,b) Primary cells in adherent culture and sphere formation in suspension culture with the expression of GFP. (c) RT-qPCR analysis of stemness markers in primary tumor cells. The data were analyzed using ordinary one-way ANOVA multiple comparisons and presented as the mean ± standard deviation ****P < 0.0001, ***P < 0.001, **P < 0.01, *P < 0.05.