Figure 6

PI3K signaling pathway was activated in primary culture cells. (a) rt-qPCR analysis of Pik3ca, Pik3cb, Pik3cg, Pik3r1, Pik3r5, Pik3r6 and PTEN expression. (b) Western blotting analysis of the AKT expression and phosphorylation. (c) Western blotting analysis of the β-catenin, Erk1/2 and phosphorylated Erk1/2.