Figure 4
Flow cytometry analysis of surface display of Ag85B-ESAT6-DC(AgE6-DC) in eight species of Lactobacillus. Cells were harvested 3 hours after induction with 100 ng/µl of SppIP. pEV, strains harboring empty vector (dotted black lines); cyt, strains harboring a vector for intracellular expression (yellow); lipo (blue), cwa2 (red) and LysM (green), strains harboring various plasmids for expression of surface-anchored antigen. The results for “lipo” have been published previously, where they were reported as MFI, i.e., medians of fluorescence intensity30. The data presented are from one representative experiment, out of at least three experiments in total.
