Figure 1

The expression of hepatic Angptl8 mRNA and protein were increased by refeeding after fasting. (a) After a 12 h fast, 20-week-old male mice were re-fed ad libitum and euthanised after 60 and 240 min. Total RNA of the liver was isolated and subjected to qPCR to quantify Angptl8 mRNA levels. Data represent mean ± SEM from 5 mice at each time point from triplicate PCR samples. The experiment was repeated once with similar results. Asterisks indicate significant differences from fasting Angptl8 expression levels (***p < 0.001). (b) Whole cell lysates of the liver were prepared from three different mice at each time point and subjected to immunoblotting using anti-Angptl8 and anti-cyclophilin antibodies. The Angptl8 and the cyclophilin blots were conducted by re-probing the same parts of the same membrane.