Figure 2

Promoter deletion analyses of mouse Angptl8 gene in liver- or non-liver-derived cell lines and mouse primary hepatocytes. (a) Schematic representation of mouse Angptl8 gene promoter constructs. The serially deleted promoter regions (−2291/+101, −1508/+101, −966/+101, −309/+101, and −59/+101) were ligated to the pGL4.10 luciferase reporter vector (Luc). The position of the consensus-binding motif for hepatocyte nuclear factor-1 (HNF-1) at −84/−68 (GGTTAACCATTGACCAG) is indicated as oblique boxes. These promoter constructs were transfected to mouse hepatoma-derived Hepa1–6 cells (b), human hepatoma-derived HepG2 cells (c), mouse primary hepatocytes (d) or HeLa cells originated from human uterine cervical cancer (e). The luciferase activity of the −59/+101 construct was set as 1 and data represent mean ± SEM from three independent experiments with triplicate determinations. Asterisks indicate significant differences from the activity of −59/+101 construct (*p < 0.05, **p < 0.01 or ***p < 0.001).