Figure 6

(a,b) The mRNA levels of Hnf-1α, but not Hnf-1β, in the liver were significantly increased after refeeding. After a 12 h fast, mice were refed ad libitum and sacrificed after 60 and 240 min. Total RNA of the liver was isolated and subjected to qPCR to quantify mRNA levels of Hnf-1α or β. Data represent mean ± SEM from 5 mice at each time point from triplicate PCR samples and the experiment was repeated once with similar result. Asterisks indicate the significant difference from mRNA levels in the liver of fasted mice (***p < 0.001). (c) Hepatic Hnf-1 protein levels were increased by refeeding. After a 12 h fast, mice were refed ad libitum and euthanised after 60 and 240 min. Whole cell lysates of the liver were prepared from three different mice at each time point and subjected to immunoblotting using anti-Hnf-1 and anti-cyclophilin antibodies. The blot of Hnf-1 and the blot of cyclophilin were cropped from different parts of the same membrane.