Figure 2

Distribution of nerve fibers, B cells, and DCs in splenic nodule/marginal zone (A), PALS (B), and red pulp (C,D) of a C57BL/6 mouse spleen. Antibodies against NF-H (red), B220 (green), and CD11c (blue) detect mainly nerve fibers, B cells, and DCs, respectively. The cyan arrows indicate B220⁺ B cells closely associated with nerve fibers. B220^-CD11c⁺ DCs closely apposed to nerve fibers were shown by white arrows. The yellow arrows indicate B220⁺CD11c⁺ DCs closely associated with nerve fibers. (B) Images in the second row (high-resolution views of the image cropped from the first row) show close associations (indicated by white circles) with nerve endings (appearing as red dots) and immune cells in PALS. (C) Trabecular plexus travels along the trabecula. Each micrograph is a maximal intensity projection of a Z-Stack. Stack size: 6.0 µm; optical slice interval: 0.50 µm. BV: blood vessel; MZ: marginal zone; SN: splenic nodule; CA: central artery; PALS: periarteriolar lymphoid sheath; T: trabecula; TX: trabecular plexus; Objective lens: 40×; Scale bar: 20 µm.