Table 1 Specificities and sources of primary and secondary antibodies.
Target [Cata. no.] | Conjugate | Species and isotype | Main cells labeled | Dilution | Company |
|---|---|---|---|---|---|
CD11c [60002] | — | Armenian Hamster monoclonal IgG | Dendritic cell | 1:500 | STEMCELL Technologies (Tullamarine, Australia) |
B220 (CD45R) [103202] | — | Rat monoclonal IgG | B cell | 1:300 | Australian Biosearch (Karrinyup, Australia) |
CD31 [102402] | — | Rat monoclonal IgG | Blood vessel endothelial cells | 1:300 | Australian Biosearch |
CD4 [100506] | — | Rat monoclonal IgG | CD4+ Thymocytes | 1:300 | Australian Biosearch |
CD8a [100802] | — | Rat monoclonal IgG | CD8a+ Thymocytes | 1:300 | Australian Biosearch |
Mac1(CD11b) [100302] | — | Rat monoclonal IgG | Macrophages | 1:300 | Australian Biosearch |
F4/80 [123102] | — | Rat monoclonal IgG | Macrophages | 1:300 | Australian Biosearch |
Neurofilament 200 (NF-H) [N4142] | — | Rabbit polyclonal | Neuronal marker | 1:2000 | Sigma (St. Louis, Missouri, US) |
Rabbit IgG H&L [ab150078] | Alexa Fluor 555 | Goat polyclonal | — | 1:1000 | Abcam Australia (Melbourne, Australia) |
Armenian Hamster IgG H&L [ab173004] | Alexa Fluor 647 | Goat polyclonal | — | 1:1000 | Abcam Australia |
Rat IgG H&L [ab150157] | Alexa Fluor 488 | Goat polyclonal | — | 1:1000 | Abcam Australia |
