Figure 5

Thermal stability of SsRidA-1 and SsRidA-2. The fraction of unfolded protein was determined from the changes in CD ellipticity at 220 nm. Upper panel: temperature-dependence of the CD signal of SsRidA-1 and SsRidA-2 in physiological saline solution (0.9% NaCl). Lower panel: temperature-dependence of the CD signal of SsRidA-1 recorded in the presence of increasing concentration of urea. Inset: linear fitting of the calculated Tm values as a function of urea concentration.