Figure 2

Endothelial expression of apelin and APJ in db/db & db/db-204^−/− mice. (a) Immunostaining of aortic sections showing total (T) and endothelial (E) apelin in db/db-204^−/− mice (magnification ×63, Scale bar; 10 µm). (b,c) Quantification of total (b) and endothelial (c) apelin in ‘a’. db/+: n = 10(4), db/+−204^−/−: n = 10(4), db/db: n = 7(3) db/db-204^−/−: n = 8(3). (d) Immunostaining of aortic sections showing total and endothelial APJ in db/db-204^−/− mice (magnification ×63, Scale bar; 10 µm). (e,f) Quantification of total (e) and endothelial (f) APJ in ‘d’. db/+: n = 14(4), db/+−204^−/−: n = 14(4), db/db: n = 11(3), db/db-204^−/−: n = 12(3). The experimental replicate is shown as “n(N)” where ‘n’ represents the number of images and ‘N’ represents the number of mice. ns>0.05, *p < 0.05, and ***p < 0.001 vs. indicated group. The data is shown as mean, and the error bar represents s.e.m. One-way analysis of variance (ANOVA) followed by Tukey’s test was performed. vWF, von-Willebrand factor.