Figure 2
From: Robust detection of undifferentiated iPSC among differentiated cells
Identification of candidate genes for detecting undifferentiated iPSC during hepatic differentiation. (a) Single-cell RNA sequence analysis identified novel candidate genes. Upper illustration and color column represents differentiation lineages from undifferentiated iPSC to mature hepatocyte (MH). DE: definitive endoderm, HE: hepatic endoderm, IH: immature hepatocyte. (b) qPCR analysis of marker gene candidates during hepatic differentiation. Down regulation of gene expressions during hepatic differentiation. The relative expression levels were normalized by the amount of 18S rRNA in each sample.
